Do not heat, dilute or add reducing agent before loading.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25☌), 2% SDS, 0.2mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol). The ladder is supplied in gel loading buffer and is ready to use. Our Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.Ģ. Monitoring protein transfer onto membranes after western blotting.ģ. Sizing of proteins on SDS-polyacrylamide gels and western blots.Ħ7 mM Tris-H 3PO 4, pH7.5, 5 mM EDTA, 2% SDS, 33% Glycerol, 0.02% proclin300.Enzo's Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 240 kDa. The ladder contains one orange reference band at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.ġ. The protein marker is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.This prestained protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Prestained Protein Marker is a mixture of ten blue, orange or green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Membrane-compatible: colored bands transfer to membranes for Western blotting.Two reference bands: orange at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.Stripping buffer alters the chemiluminescent functionality of the ladder, resulting in. Note: The WesternSure Ladder is not recommended for use when stripping and re-probing Western blots. Quality tested: each lot evaluated by SDS-PAGE and Western blotting. The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection.They are detected by incubating the blot with Europium-labeled streptavidin and they show as light colored bands when read with the ScanLater Western Blot Detection Cartridge. Sharp bands: color-coded bands of similar intensity for easy visualization. The ScanLater Western Blot Protein Ladder consists of seven biotinylated recombinant proteins at 10, 20, 40, 50, 80, 100 and 140 kD.
The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. Ready-to-use: supplied in a loading buffer for direct loading on gels no need to boil. Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting.My experimental protein size is 124kDA and I choose Beta Actin as a loading control which is small. Size range: 10 proteins spanning from 10 to 180 kDa. I am a beginner of western blot and from some days I have been facing a lot of problems.